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A high-throughput solid phase screening method has been developed for alanine racemase. Conversion of an l-amino acid to the d-amino acid is detected by a d-amino acid oxidase, which is utilised as a reporter enzyme along with horseradish peroxidase and a colourimetric dye. This system has successfully been used to identify a mutant with increased activity towards arginine. Kinetic analysis and modelling...
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