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Natural abundance deuterium 2D‐NMR (NAD 2D‐NMR) experiments in oriented solutions of polypeptides have been used to successfully resolve almost all inequivalent deuterium sites of long‐chain saturated fatty acid methyl esters, a crucial step for establishing their natural isotopic profile. Theoretical prediction of quadrupolar splittings confirms the spectral assignments and demonstrates the key role...
The overall and detailed elucidation (including the stereochemical aspects) of enzymatic mechanisms requires the access to all reliable information related to the natural isotopic fractionation of both precursors and products. Natural abundance deuterium (NAD) 2D‐NMR experiments in polypeptide liquid‐crystalline solutions are a new, suitable tool for analyzing site‐specific deuterium isotopic distribution...
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