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Nicotine or nornicotine enriched with stable isotopes in either the N′-methyl group or the pyrrolidine-N were fed to Nicotiana plumbaginifolia suspension cell cultures that do not form endogenous nicotine. The metabolism of these compounds was investigated by analysing the incorporation of isotope into other alkaloids using gas chromatography–mass spectroscopy (GC–MS). Nicotine metabolism primarily...
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