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The Escherichia coli lacZ gene encoding β‐galactosidase is a widely used reporter, but few synthetic substrates are available for detecting its activity with single‐cell resolution in living samples. Our recently reported fluorogenic substrate SPiDER‐βGal is suitable for this purpose, but its hydrolysis product shows green fluorescence emission, and a red‐shifted analogue is therefore required for...
The LacZ gene, which encodes Escherichia coli β‐galactosidase, is widely used as a marker for cells with targeted gene expression or disruption. However, it has been difficult to detect lacZ‐positive cells in living organisms or tissues at single‐cell resolution, limiting the utility of existing lacZ reporters. Herein we present a newly developed fluorogenic β‐galactosidase substrate suitable for...
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