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An improved method for expression of poly-β-hydroxyalkanoate (PHA) synthase fromAlcaligenes eutrophushas been developed using the baculovirusAutographa californicanuclear polyhedrosis virus (AcMNPV) in BTI-TN-5B1-4Trichoplusia nicells which results in high level production of active PHA synthase. Confirmation of expression of authentic PHA synthase was obtained by Western analysis which also revealed...
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