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BACKGROUNDThe coenzyme NAD+ (nicotinamide adenine dinucleotide) is commonly used in biocatalytic oxidations catalysed by the enzyme alcohol dehydrogenase (ADH). As the price of the coenzyme NAD+ is extremely high, it is essential to regenerate the reduced form of the coenzyme back into the oxidized form. In this work the regeneration of the coenzyme NAD+ was carried out in a microreactor by reversible oxidation of ethanol to acetaldehyde with the ADH enzyme...
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