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The Mucor miehei lipase, Lipozyme, has been found to be vulnerable to temperature-induced deactivation. This thermodeactivation is further aggravated by exposure to butanol. The deleterious effect of the latter on the lipase is a function of its concentration in the aqueous phase containing the enzyme. At 50 o C, it was found that a crucial butanol concentration (0.81 m) exists, up to which...
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