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An exquisitely specific “affinity clamp” with nanomolar affinities was recently engineered via directed evolution strategy, which is composed of the human Erbin-PDZ domain and the fibronectin type III (FN3) domain. This high-specificity affinity clamp showed distinct binding affinity to two p120-related catenin peptides, δ-catenin and ARVCF. To investigate the interactions between the affinity clamp...
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