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Rotational-echo double-resonance (REDOR) solid-state NMR is applied to probe the membrane locations of specific residues of membrane proteins. Couplings are measured between protein 13 CO nuclei and membrane lipid or cholesterol 2 H and 31 P nuclei. Specific 13 CO labeling is used to enable unambiguous assignment and 2 H labeling covers a small region of the...
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