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A method is described for extracting the total RNA of thermophilic bacteria, modified from the original protocol that was developed by Hofle [7] and used successfully on mesophilic strains. The modifications consist of the use of guanidium thiocyanate as a potent protein inhibitor and sarcosyl as detergent coupled to phenol/chloroform for lysis of cell. Another important step is the use of Qiagen...
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