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Klicken und analysieren: Die im Titel genannte Sonde (SF‐Eu), die durch Klickreaktion eines Sulfonylfluorids mit einer Azido‐Einheit synthetisiert wurde, reagiert spezifisch mit Serin im aktiven Zentrum von Serinproteasen (SPs). Die Kombination der Methode mit 153Eu‐Isotopenverdünnungs‐ICP/MS ermöglicht die absolute Proteinquantifizierung aktiver SPs in biologischen Proben mit nur einem einzigen 153...
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