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Here, we provide a protocol for reliable isolation and subculture of putative mesenchymal stem cells from mice colons. This method provides a good approach to cultivate and characterize putative colonic mesenchymal stem cells (cMSCs). A high purity of cMSCs can be obtained according to this protocol. The whole isolation processes of cMSCs take about 2 h with two important digestion steps involved...
Catalases are essential components of the cellular equipment used to cope with oxidative stress. The monofunctional catalase KatP was purified from Pigmentiphaga sp. using ammonium sulfate precipitation (ASP), diethylaminoethyl ion exchange chromatography (IEC), and hydrophobic interaction chromatography (HIC). The purified catalase formed polymer with an estimated monomer molecular mass of 54kDa,...
We have studied the synthesis method of D-glucosamine Complexes with Lead(II), the main factors affecting the reaction and ideal reaction conditions. By the mass spectrometry and IR Spectrum analysis of the complexes, we understand the reaction ratio and coordination situation gradually, and determine the existing of the bond of N-Pb and O-Pb. At last, we Infer the Structure of the Complexes.
Phenylephrine (i.e. PHE) and chlorprothixene (i.e. CPT), two effective and important antipsychotic drugs with low redox activity, were found generating an irreversible anodic peak at about +0.89V (vs. SCE) and +1.04V in 0.05M HAc–NaAc (pH 5.0) or NH 2 CH 2 COOH–HCl (pH 2.4) buffer solution at poly(4-aminobenzene sulfonic acid) modified glassy carbon electrode (i.e. poly(4-ABSA)/GC),...
Clozapine, an effective antipsychotic drug, was found generating a pair of redox peaks at about 0.33–0.4V (versus SCE) at 16-mercaptohexadecanoic acid (i.e. MHA) self-assembled monolayer (SAM) modified gold electrode (i.e. MHA/Au) in 0.05molL −1 Tris–HCl (pH 8.1) buffer solution. Sensitive and quantitative measurement of clozapine based on anodic peak was established under optimum conditions...
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