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A sensitive and selective method for the determination of dopamine is described. Dopamine was oxidized by mercury(II) nitrate and the oxidation product was condensed with ethylene diamine to form a quinoxaline derivative which was strongly fluorescent. The measurement was carried out at 447nm with excitation at 393nm. Effects of pH, oxidants, and foreign ions on the determination of dopamine were...
The property of dopamine in acid-alkaline medium was studied by spectrofluorimetry and spectrophotometry. Effects of organic solvents on dopamine fluorescence spectra were examined and the reason was discussed. Among the organic solvents tested, methanol was selected as sensitizing reagent. A fluorescence quenching reaction took place in basic medium. Mechanism of fluorescence quenching was discussed...
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