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An immunosensor based on surface plasmon resonance (SPR) was used for the detection of a low-molecular-weight analyte, domoic acid (DA) (MW 310g/mol), using an inhibition assay. A novel surface functionalization method was developed to chemically immobilize DA molecules onto a gold-coated SPR chip functionalized with mixed oligo(ethylene glycol) (OEG) self-assembled monolayers (SAMs), where the long-chain...
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