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In this study, we investigated the effect of flow perfusion culture on the mineralization of co-cultures of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs). Osteogenically precultured hMSCs were seeded onto electrospun scaffolds in monoculture or a 1:1 ratio with HUVECs, cultured for 7 or 14 days in osteogenic medium under static or flow perfusion conditions,...
The combination of reduced oxygen tension and flow perfusion bioreactor culture is investigated for its effect on the proliferation, glycosaminoglycan production, and chondrogenic gene expression of bovine articular chondrocytes on porous polymer scaffolds. It was hypothesized that the combination of such factors would more closely replicate the in situ environment of these cells, leading to improvements...
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