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In the green alga Chlamydomonas reinhardtii, the cytosolic GLUTATHIONE PEROXIDASE 5 gene (GPX5) is known to be transcriptionally up-regulated in response to singlet oxygen (1O2). As demonstrated by previous studies, fusion of the promoter region of GPX5 to the ARYLSULFATASE 2 gene (ARS2) creates an effective reporter system that can be used to monitor 1O2-driven GPX5 expression. This system was also...
To increase our knowledge of anaphase promoting complex (APC/C) function during plant development, we characterized an Arabidopsis thaliana T-DNA-insertion line where the T-DNA fell within the 5′ regulatory region of the APC10 gene. The insert disrupted endogenous expression, resulting in overexpression of APC10 mRNA from the T-DNA- internal CaMV 35S promoter, and increased APC10 protein. Overexpression...
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