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The invention provides alpha-1-antichymotrypsin and protein preparations comprising human alpha-1-antichymotrypsin produced by E. coli cells transformed with a DNA sequence encoding human alpha-1-antichymotrypsin. The invention also provides methods for producing alpha-1-antichymotrypsin. The invention further provides analogues of alpha-1-antichymotrypsin that exhibit antichymotrypsin, anti-trypsin...
The variants of Escherichia coli pyrophosphatase carrying the substitutions Glu20→Asp, His136→Gln or His140→Gln are inactivated, in contrast to the wild-type enzyme, at temperatures below 25°C: their activity measured at 25°C decreases with decreasing the temperature of the stock enzyme solution. The inactivation is completely reversible and is explained by cold-induced dissociation of these hexameric...
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