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To observe the formation process of compound I in horseradish peroxidase (HRP), we developed a new freeze-quench device with ∼200μs of the mixing-to-freezing time interval and observed the reaction between HRP and hydrogen peroxide (H2O2). The developed device consists of a submillisecond solution mixer and rotating copper or silver plates cooled at 77K; it freezes the small droplets of mixed solution...
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