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In this work the dimerization process of the minor allelic form of human alanine glyoxylate aminotransferase, a pyridoxal 5′-phosphate enzyme, was investigated. Bioinformatic analyses followed by site-directed mutagenesis, size exclusion chromatography and catalytic activity experiments allowed us to identify Arg118, Phe238 and Phe240 as interfacial residues not essential for transaminase activity...
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