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The first SH3 domain (SH3.1) of Nckα specifically recognizes the proline-rich region of CD3ɛ, a subunit of the T cell receptor complex. We have solved the NMR structure of Nckα SH3.1 that shows the characteristic SH3 fold consisting of two antiparallel β-sheets tightly packed against each other. According to chemical shift mapping analysis, a peptide encompassing residues 150–166 of CD3ɛ binds at...
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