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The effect of point mutation in the sequence 316 TWLE 319 , which occurs in the extracellular loop flanking the third (M3) and the fourth (M4) transmembrane segment (L3/4) of the Na + ,K + -ATPase α-subunit, was examined. Mutation of Glu 319 to Asp yielded an enzyme with full activity, whereas substituting Glu 319 to Ala resulted in a severe loss of...
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