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BackgroundThe ability to assess gene function is essential for understanding biological processes. Currently, RNA interference (RNAi) is the only technique available to assess gene function in planarians, in which it has been induced by means of injection of double‐stranded RNA (dsRNA), soaking, or ingestion of bacteria expressing dsRNA.
ResultsWe describe a simple and robust RNAi protocol, involving...
COVER PHOTOGRAPH: Low magnification image of planarian flatworms (Schmidtea mediterranea) fed with liver paste containing either red, green, yellow, or blue food dye. Uptake of colored liver paste containing in vitro synthesized double‐stranded RNA is readily visualized in the planarian gut. Ingestion and processing of double‐stranded RNA in the planarian gut leads to systemic RNA‐interference. Image...
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