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The functional interplay between tBID and phospholipids was investigated in this study. The binding of tBID to model membranes was increased by an incorporation of phosphatidylserine (PS) into the liposomes. Using limited proteolysis and mass spectrometry, two peptide regions, which correspond to Ser100–Arg114 and His89–Arg114 in BID, revealed the specific PS-binding site. tBID also decreased the...
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