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Flavin-binding, Kelch repeat, F-box (FKF1) protein is a photoreceptor to regulate flowering of Arabidopsis. The protein has a light, oxygen and voltage (LOV)-sensing domain binding a flavin mononucleotide. The photo-activation of the domain is an indispensable step to initiate the cellular signaling for flowering. In the present study, a LOV-containing polypeptide of FKF1 was prepared by an overexpression...
The dimeric molecular structure of pea phytochrome (monomer molecular mass 114 kDa) in the red-light-absorbing form was studied with reference to the structures of its molecular domains in a monomer (an N-terminal 59-kDa chromophoric domain and a C-terminal 55-kDa nonchromophoric domain) using small-angle X-ray scattering, and a 'four-leaved shape' model is proposed. The propriety of the model was...
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