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Two [NiFe] hydrogenases extracted from the hyperthermophilic bacterium Aquifex aeolicus (Aa) are studied at a pyrolytic graphite electrode using cyclic voltammetry. The two hydrogenases have been shown to exhibit very similar behavior. They are able to catalyze under a nitrogen or a hydrogen atmosphere, the direct hydrogen production from protons and the reverse reaction of hydrogen oxidation directly...
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