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Stationary phase optimized liquid chromatography (POPLC) has been applied to the separation of oligopeptides. The retention factors and theoretical plate numbers of 13 peptides were determined on five different stationary phases. Based on these values, an optimal stationary phase composition of 250mm total length consisting of 3 segments of 20mm octadecyl silica, 10mm phenyl silica and 220mm embedded...
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