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The capacity of microsomes from aminopyrine-induced Jerusalem artichoke (Helianthus tuberosus L.) to oxidize saturated and unsaturated fatty acids has been investigated using lauric acid and a series of unsaturated lauric acid analogs (7-, 8-, 9- and 10-dodecenoic acids) as radiolabeled substrates. In the presence of NADH, lauric acid was mono-hydroxylated principally at carbon 9. Steric analysis...
A new epoxygenase, differing in substrate and stereospecificity from those previously described in mammals, bacteria and Vicia faba, has been characterized in higher plants. A microsomal fraction from Jerusalem artichoke tubers which catalyses in-chain monohydroxylation of lauric acid has now been found to convert synthetic 9-dodecenoic acid (9-DDNA) to 9,10-epoxylauric acid. Epoxidation of the cis...
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