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The HTRF (homogeneous time-resolved fluorescence) Transcreener ADP assay is a new kinase assay technology marketed by Cis-Bio International (Bagnols-Cèze, France). It measures kinase activity by detecting the formation of ADP using a monoclonal antibody and HTRF detection principles. In this article, we compare this technology with a standard HTRF kinase assay using EGFR [L858R/T790M] mutant enzyme...
The rapidly growing interest in kinases as potential targets for therapeutic intervention has prompted the development of many kinase assay technologies. One exciting example is homogeneous time-resolved fluorescence (HTRF). An HTRF assay utilizes the signal generated by the fluorescence resonance energy transfer between donor and acceptor molecules in close proximity. Dual-wavelength detection helps...
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