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Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1–393), and its C-terminal fragment, p53(320–393), with long (∼3000 bp) dsDNA in linear and supercoiled (∣ΔLk∣≈4–6) forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA–protein filament, in which two DNA duplexes...
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