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The effect of oxidants on Ca2+ movement in osteoblasts is unclear. In this study, we show that 4,4-dithiodipyridine (4,4-DTDP), a reactive disulphide that mobilizes Ca2+ in muscle, induces an increase in cytoplasmic free-Ca2+ concentrations ([Ca2+]i) in MG63 human osteosarcoma cells loaded with the Ca2+-sensitive dye fura-2. 4,4-DTDP acted in a concentration-dependent manner with an EC50 of 10M. Removing...
2,2'-dithiodipyridine (2,2'-DTDP), a reactive disulphide that mobilizes Ca 2+ in muscle, induced an increase in cytoplasmic free Ca 2+ concentrations ([Ca 2+ ] i ) in MG63 human osteosarcoma cells loaded with the Ca 2+ -sensitive dye fura-2. 2,2'-DTDP acted in a concentration-independent manner with an EC 50 of 50 μM. The Ca...
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