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UV and IR laser ablation under MALDI conditions is described for two typical solid UV and IR matrices. 2,5-Dihydroxy benzoic acid (2,5-DHB) as a UV and IR matrix and succinic acid (SA) as an IR matrix were investigated systematically by light and electron microscopy, and by mass spectrometry. Large single crystals of 2,5-DHB and SA with and without protein incorporated were used for the experiments...
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