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Conditions for the optimal expression of the human CYP1B1 hemoprotein in Escherichia coli have been investigated. CYP1B1 cDNA was prepared from a retinal cDNA template and used to generate cDNA fragments with modified 5'-sequences reported to allow enhanced expression in E. coli DH5α. Plasmids were constructed, using the pCWori+ expression vector and were used to examine necessity for thiamine, δ-aminolevulinic...
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