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The source of retrieval about stage's design knowledge base is text script, and text processing has become the key technologies about obtaining related information from script. This paper proposes a method of extracting from the script in the keyword categories by analyzing the characteristics of the script, furthermore, develop technical solutions for analysis of script's emotion by experiment.
this paper reports about the formal analysis and verification of a Multimedia Messaging Service Protocol (MMS) used by NOKIA in its products. We started with the Timed Automata models of the MMS protocol, and then we performed verifications by model-checking using the UPPAAL. The Timed Automata models of the MMS were modeled with the UPPAAL's Editor and simulated its trace by UPPAAL's Simulator, and...
Unlike other efforts on supporting triples of higher orders of magnitude in a centralized triplestore, we believe, as the semantic Web evolves, the unstructured P2P paradigm may better fit with the application scenario of triplestores used by individuals in the future. We designed an unstructured P2P RDF triplestore, focusing on its efficient query routing mechanism, and demonstrated that it can deliver...
A long circulation paclitaxel liposome was prepared by dispersion of film method. The size of liposomes was around 207 nm and drug encapsulation efficiency was 96.5%. The PEGylated paclitaxel liposomes were very stable when stored in PBS, pH 6.5 at 4degC. After 22 days, leakage of drug from liposomes was only 6%. In vivo biodistribution of paclitaxel liposomes and free paclitaxel were compared in...
A new approach for detection cleavage product of restriction enzyme was reported based on molecular-beacon- ligation system. The molecular beacon, designed in such a way that one-half of its loop is complementary to cleavage product, is used to monitor ligation process of nucleic acids complex and to convert directly cleavage product information into fluorescence signal. The method is fast, simple...
A one-dimensional addressable beads array for nucleic acids detection is presented in this paper. Molecular beacons (MBs) modified porous silica micro-beads were deposited along a micro-channel in sequence. This MB-functional beads array cooperating with microfluidic control allows multiple nucleic acid targets detection simultaneously with only once samples injection, a rapid dynamic hybridization...
A simple and fast method for separation of cytochrome C (Cyt-C) has been reported based on the electrostatic adsorption between silica coated magnetic nanoparticles (SMNPs) and Cyt-C. When the solution pH is above 6.0, but below the isoelectric point of Cyt-C (pi = 10.6), SMNPs possess zeta potential below -30 mV and can electrically adsorb positively charged Cyt-C to form SMNPs-Cyt-C complex. The...
Here some progresses of core-shell nanoparticles (NPs) for biomedical applications have been reported. A new synthesis mechanism for preparation of core-shell NPs by using water-in-oil (W/O) microemulsion technique has been investigated. And the applications of the core-shell NPs on cell recognition, oligonucleotide detection, single bacterial detection, pH nano-sensor for single cell, DNA recovery...
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