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The effects of pH and ionic strength (salt concentration) on transmission of single protein human serum albumin (HSA) and human immunoglobulins (HIgG) through a 100 kDa molecular weight cut-off (MWCO) polyethersulfone membrane have been studied using a pulsed sample injection technique. Experimental results obtained clearly demonstrate the effectiveness of changing solution pH and ionic strength optimisation...
This paper discusses the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration (30kDa MWCO, polysulphone membrane). Lysozyme is preferentially transmitted through the membrane while the membrane largely retains other egg white proteins. Improvement in system hydrodynamics resulted in an increase in permeate flux while lysozyme transmission remained unaffected, leading...
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