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A novel on-line liquid chromatography–photodiode array detection–mass spectrometry (LC–DAD–MS) system was established with restricted-access media (RAM) pre-column and dilution line combined with a column-switching valve. The serum samples were injected directly onto pre-column under diluted condition by dilution line. After elution of proteins in the serum, the analytes were backflushed onto an ODS...
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