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Antibody engineering technology has the potential to provide artificial antibodies with higher performance than conventional antibodies. Filamentous phage particles are often used to express a vast diversity of mutated antibody fragments from which clones displaying improved fragments can be isolated. We recently showed that hapten–biotin conjugates, combined via a linker involving a reductively cleavable...
A single-chain Fv fragment (scFv) against estradiol-17β (E 2 ) was generated to begin the construction of a library of various mutated anti-steroid antibodies with an improved affinity and/or specificity. A hybridoma clone secreting a specific anti-E 2 antibody (Ab#E4-4) was established by the cell fusion using splenocytes from a mouse immunized with an immunogenic E 2 -carrier...
The engineering of hapten-specific antibodies with affinity constant higher (K a values>10 10 M −1 ) than those of conventional antibodies promises hapten immunoassays exhibiting sub-femtomole range sensitivity, based on the conventional competitive assay principle. Here we report a simple method to select phage particles displaying anti-hapten antibody fragments with exceptionally...
Single-chain Fv fragments (scFvs) against a corticosteroid, 11-deoxycortisol (11-DC), have been generated as a template antibody fragment from which a comprehensive mutated antibody library containing various anti-steroid antibodies could be constructed. The cDNAs encoding variable heavy (V H ) and light (V L ) domains of a mouse anti-11-DC antibody (CET-M8), were amplified by RT-PCR,...
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