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A membrane‐receptor‐analysis method termed ligand dilution analysis, which combines the concepts of single‐molecule localization and random sampling, is reported by Yifan Lyu, Jianhui Jiang, Weihong Tan et al. in their Research Article (e202215387). Aptamer binding characterization, including receptor density calculation, single‐molecule colocalization, binding site determination, motion and fluctuation...
Cell‐specific aptamers offer a powerful tool to study membrane receptors at the single‐molecule level. Most target receptors of aptamers are highly expressed on the cell surface, but difficult to analyze in situ because of dense distribution and fast velocity. Therefore, we herein propose a random sampling‐based analysis strategy termed ligand dilution analysis (LDA) for easily implemented aptamer‐based...
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