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Although the lipase of Geotrichum candidum has been extensively reported, little attention has been focused on molecular genetic and biochemical characterizations of Galactomyces geotrichum lipases. A lipase gene from G. geotrichum Y05 was cloned from both genomic DNA and cDNA sources. Nucleotide sequencing revealed that the ggl gene has an ORF of 1692bp without any introns, encoding a protein of...
The amplification of variable regions (Fv) of immunoglobulins (Ig) becomes a major challenge in cloning antibody genes either from hybridoma cell lines or splenic B cells. When we amplified the heavy-chain variable regions (VH) gene from one hybridoma cell line which were not amplified successfully under conventional protocols, a novel method was developed to design the degenerated primer of immunoglobulin...
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