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A novel lipase gene, lipJ08, was cloned from Candida rugosa ATCC14830, along with the already reported five lipase genes (lip1–lip5). Nucleotide sequencing indicated that the lipJ08 gene contains a 1650 bp open reading frame (ORF) without introns. The deduced amino acid sequence corresponds to 534 amino acid residues, including a putative signal sequence of 15 amino acid residues. Seventeen of the...
From the N-terminal amino acid sequence of the lipase from Aspergillus niger F044, a potential homologous gene A84689 to the lipanl (the gene encoding the lipase from Aspergillus niger F044) was identified. A pair of primers were designed according to the nucleotide sequence of A84689, and the lipanl was cloned by PCR. Nucleotide sequencing revealed that the lipanl has an ORF of 1,044 bp, containing...
The amplification of variable regions (Fv) of immunoglobulins (Ig) becomes a major challenge in cloning antibody genes either from hybridoma cell lines or splenic B cells. When we amplified the heavy-chain variable regions (VH) gene from one hybridoma cell line which were not amplified successfully under conventional protocols, a novel method was developed to design the degenerated primer of immunoglobulin...
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