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We report free‐solution microchip electrophoresis performed at elevated temperatures and high separation field strengths. We used microfluidic devices with 11 cm long separation channels to conduct separations at temperatures between 22 (ambient) and 45°C and field strengths from 100 to 1000 V/cm. To evaluate separation performance, N‐glycans were used as a model system and labeled with 8‐aminopyrene‐1,3,6‐trisulfonic...
We designed and fabricated microfluidic devices with serpentine separation channels and asymmetrically tapered turns, thus allowing high efficiency separations and minimizing band broadening associated with the “racetrack” effect. We evaluated the performance of these devices by measuring the variation in separation efficiency with separation length, electric field strength, taper ratio of the turns,...
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