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A novel cytokine assay has been designed using a cellular chip by combining a collagen gel embedded cell culture technique with scanning electrochemical microscopy–enzyme linked immunosorbent assay (SECM–ELISA). An array of cell–collagen gel mixture (2μL) was spotted on an antibody-coated chip and incubated for 0.5–24h. The very small trace amounts of cytokines produced by the activated leukocytes...
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