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The advent of ionization methods capable of forming gaseous oligonucleotide ions has made mass spectrometry a viable tool for structural studies of nucleic acids. Structural information regarding the primary sequence of a nucleic acid and the nature of its nucleobases via mass spectrometry has relied primarily on the dissociation behavior of ions formed by one or more of the available ionization methods...
A series of anions derived from electrospray ionization of small oligonucleotides, each consisting of a single nucleobase (adenine, thymine, cytidine or guanine), have been subjected to ion trap tandem mass spectrometry. The data set allows for comparisons of dissociation behavior as functions of parent ion charge, oligomer length, and nucleobase identity. Parent ion charge is seen to play a major...
Multiply-charged cations derived from the positive ion electrospray of the oligonucleotide 5 -d(CTTAGCGCTAAG)-3 were subjected to reactions with the (M - CF 3 ) - and (M - F ) - anions derived from perfluoro-1,3-dimethylcyclohexane in a quadrupole ion trap. Both single strand ions and double strand ions formed from the self-complementary oligonucleotide were...
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