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The cleavage of fluorescence-labeled M13DNA (7250 bp) usingHaeIII,HgaI,BsmAI, andBspMI was analyzed by fluorescence correlation spectroscopy (FCS) in a small volume (1.5 × 10−15liters). The digestion process can be monitored by the decrease in amplitude of the fluorescence correlation function while the original DNA molecule is divided into several fragments by the enzymes. To analyze this reaction...
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