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Phenazine compounds have been used as simple organic catalysts for the rapid oxidation of NADH. Although positively charged phenazine compounds are highly water‐soluble, their uncharged, reduced forms are not. We report the use of a zwitterionic phenazine compound, 3‐(1‐methoxyphenazin‐5‐ium‐5‐yl)propane‐1‐sulfonate (mPPS), as an organic catalyst and electron mediator for the electrochemical oxidation...
Rapid and sensitive electrochemical detection of NADH, NADH‐dependent enzymes, and relevant metabolites requires a redox enzyme along with an electron mediator. Preferably, the redox enzyme should be immobilized on electrode rather than dissolved in solution. In this study, to simply immobilize a redox enzyme on electrode and maintain its enzymatic activity for long time, thermostable DT‐diaphorase...
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