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In this study the reactions between nitric oxide (NO) and horseradish peroxidase (HRP) compounds I and II were investigated. The reaction between compound I and NO has biphasic kinetics with a clearly dominant initial fast phase and an apparent second-order rate constant of (7.0 ± 0.3) × 10 5 M −1 s −1 for the fast phase. The reaction of compound II and NO was found to have...
Desferrioxamine mesylate (Desferal), a transition metal ion chelator, has been used to inhibit the in vitro redox cycling of transition metal ions. ESR spectroscopy was utilized to detect and identify Desferal's one-electron oxidation product. We demonstrate that a horseradish peroxidase/H 2 O 2 system, a xanthine oxidase/hypoxanthine system, and a hydroxyl radical-generating system...
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