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A microbial biosensor consisting of a dissolved oxygen electrode modified with the genetically engineered PNP-degrader Moraxella sp. displaying organophosphorus hydrolase (OPH) on the cell surface for sensitive, selective, rapid and direct determination of p-nitrophenyl (PNP)-substituted organophosphates (OPs) is reported. Surface-expressed OPH works in tandem with the PNP oxidation machinery of the...
A novel microbial biosensor/electrode for highly selective, sensitive and rapid quantitative determination of p-nitrophenol (PNP) was developed using PNP-degrading organisms immobilized in a membrane on a dissolved oxygen electrode. Moraxella sp. specifically oxidizes PNP while consuming oxygen. A change in oxygen concentration was determined by a Clark oxygen electrode and correlated to the PNP concentration...
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