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Research involving human embryonic stem cells is an active area of investigation because of the potential role stem cells could play in therapeutic regenerative medicine. In this chapter, we provide an introduction to stem cells as well as present a microfluidic cell culture platform that may help to provide important insight into stem cell development. Since human stem cell biology is still in its...
Microfluidic channels were constructed out of polydimethylsiloxane (PDMS) and used as culture vessels for ovary cells from the fall armyworm, Spodoptera frugiperda, (Sf9). PDMS allows cells to be visually inspected and provides excellent permeability to oxygen and carbon dioxide. Cells were grown in static culture conditions and observed every 24 hours for seven days. The growth rate in microchannels...
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