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Different cis acting elements of gamma kafirin gene from Sorghum bicolor var. M 35-1 were amplified and cloned using different combination of the primers. The amplified promoter was replaced with CaMV35S promoter of vector pCMBIA-1304 and resultant vector contained β-glucuronidase (gus) gene under the control of amplified γ-kafirin promoter. The resulting fusants were then transformed in to different...
Study of the structure of high molecular weight (Mr) glutenin subunits and their interaction has been limited by the difficulty of isolating single subunits in large amounts from the complex mixture of gluten wheat endosperm. One way to overcome this problem is the expression of high Mr glutenin subunits in heterologous systems. These systems have the additional advantages that, specific gene modifications...
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