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The Arg 123 -Tyr 166 central and the Ala 190 -Gln 243 carboxyl-terminal pair of helices of apo A-I were substituted with the pair of helices of apo A-II, resulting in the apo AI[Δ(R 123 -Y 166 ), A-II(S 12 -A 75 )] and the apo A-I[Δ(A 190 -Q ...
The murine monoclonal antibody mAb-1H11, specific for malondialdehyde (MDA)-modified LDL, detected crossreacting material in human atheromatous tissue and in plasma. The mAb-1H11 based ELISA detected 0.1 mg/dl MDA-modified LDL whereas 100 mg/dl native LDL was not detected. MDA-modified LDL levels in plasma, collected in the presence of butylated hydroxytoluene to prevent in vitro oxidation, were...
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