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The aim of this study was to investigate the effects of insulin on glucose uptake in lactating bovine mammary epithelial cells (BMEC). Primary BMEC were cultured in Dulbecco’s modified Eagle’s medium/nutrient mixture F-12 and treated with different levels of insulin (0, 5, 50, and 500ng/mL) for 48h after a 24-h starvation without fetal calf serum. Compared with the control cells (0ng of insulin/mL),...
The objective was to determine if supplementing a dairy cow diet with an exogenous fibrolytic enzyme additive (Econase RDE; AB Vista, Marlborough, Wiltshire, UK) altered fermentation, pH, and microbial populations in the rumen or enteric methane (CH 4 ) emissions. In a companion study, this enzyme additive improved efficiency of fat-corrected milk production in a dose-dependent manner by up...
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